Human TIMP-2 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DTM200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL)
Sensitivity
0.064 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human TIMP-2 Quantikine ELISA Kit
The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-2 levels in cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human TIMP-2, and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.01 | 2.90 | 5.26 | 1.01 | 2.79 | 5.27 |
| Standard Deviation | 0.042 | 0.173 | 0.344 | 0.079 | 0.188 | 0.326 |
| CV% | 4.2 | 6.0 | 6.5 | 7.8 | 6.7 | 6.2 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.23 | 3.45 | 6.09 | 1.26 | 3.45 | 6.38 |
| Standard Deviation | 0.054 | 0.116 | 0.181 | 0.086 | 0.197 | 0.467 |
| CV% | 4.4 | 3.4 | 3.0 | 6.8 | 5.7 | 7.3 |
Recovery for Human TIMP-2 Quantikine ELISA Kit
The recovery of TIMP-2 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 81-108 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TIMP-2 Quantikine ELISA Kit
Human TIMP-2 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve
Human TIMP-2 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIMP-2
TIMP-2 (tissue inhibitor of metalloproteinases 2) is one of several TIMPs that form complexes with matrix metalloproteinases (MMPs) and inhibit their proteolytic activity. MMPs play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction and tissue remodeling. They also participate in many pathological processes such as arthritis, cancer and cardiovascular disease. TIMP-2 binding to pro-MMP-2 is essential for the cell surface activation of pro-MMP-2 by active MMP-14 (MT1-MMP). TIMP-2 also suppresses EGF-mediated mitogenic signaling by short-circuiting EGF R activation.
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
Additional TIMP-2 Products
Product Documents for Human TIMP-2 Quantikine ELISA Kit
Product Specific Notices for Human TIMP-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.