Human SP-D QuicKit ELISA

Name: Human SP-D QuicKit ELISA
Brand: R&D Systems
SKU: QK1920

R&D Systems, part of Bio-Techne | Catalog # QK1920

Submit a review

Product Datasheet / COA / SDS

Key Product Details

Assay Length:

80 minutes

Human SP-D QuicKit ELISA Summary:

The Quantikine QuicKit Human SP-D Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human SP-D levels in cell culture supernates, serum, plasma, and BALF. It contains recombinant human SP-D and antibodies raised against the recombinant protein. Results obtained for naturally occurring human SP-D showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural SP-D.

View all SP-D ELISA Kits »

View Full Technical Details for Human SP-D QuicKit ELISA

Technical Data
Citations / Reviews

Product Specifications for Human SP-D QuicKit ELISA

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human SP-D.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least four technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (ng/mL)	4.65	23.8	4.37	22
Standard Deviation	0.21	1.27	0.277	1.62
CV%	4.5	5.3	6.3	7.4

Recovery

The recovery of human SP-D spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.

Sample Type	Average % Recovery	Range %
Bronchoalveolar Lavage Fluid (n=2)	96	93-99
Cell Culture Media (n=4)	110	106-114
EDTA Plasma (n=2)	90	81-99
Heparin Plasma (n=2)	86	79-95
Serum (n=2)	90	77-100

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human SP-D were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human SP-D Quantikine QuicKit ELISA Standard Curve

Scientific Data Examples for Human SP-D QuicKit ELISA

	Human SP-D Quantikine QuicKit ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Item	Value
Long Name	Surfactant Pulmonary Associated Protein D
Entrez Gene IDs	6441 (Human); 20390 (Mouse)
Alternate Names	COLEC7collectin-7, Collectin 7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, SFTP4, SFTP4pulmonary surfactant-associated protein D, SFTPD, SP-D, SP-Dpulmonary surfactant apoprotein, SPD, surfactant protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary 4

Customer Reviews for Human SP-D QuicKit ELISA

There are currently no reviews for this product. Be the first to review Human SP-D QuicKit ELISA and earn rewards!

Have you used Human SP-D QuicKit ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Human SP-D QuicKit ELISA

R&D Systems, part of Bio-Techne | Catalog # QK1920

Key Product Details

View all SP-D ELISA Kits »

Product Specifications for Human SP-D QuicKit ELISA

Precision

Recovery

Linearity

Scientific Data Examples for Human SP-D QuicKit ELISA

Preparation and Storage

Background: SP-D

Customer Reviews for Human SP-D QuicKit ELISA

Product Documents for Human SP-D QuicKit ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

Stay Connected