Human Phospho-p53 (S46) DuoSet IC ELISA

Name: Human Phospho-p53 (S46) DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC1489-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC1489-2

Product Datasheet / COA / SDS

Discontinued Product

DYC1489-2 has been discontinued. View all p53 products.

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	625 - 40,000 pg/mL
Sample Types:	Cell Lysates
Human Phospho-p53 (S46) DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Phospho-p53 (S46) DuoSet IC ELISA

Summary for Human Phospho-p53 (S46) DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Human Phospho-p53 (S46) DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Phospho-p53 (S46) DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: p53

p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

Item	Value
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names	Antigen NY-CO-13, BCC7, FLJ92943, LFS1, LFS1TRP53, P53cellular tumor antigen p53, Phosphoprotein p53, TP53, TRP53, Tumor suppressor p53, p53, p53 tumor suppressor, transformation-related protein 53, tumor protein p53

Citations for Human Phospho-p53 (S46) DuoSet IC ELISA

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Human Phospho-p53 (S46) DuoSet IC ELISA

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Summary for Human Phospho-p53 (S46) DuoSet IC ELISA

Product Specifications for Human Phospho-p53 (S46) DuoSet IC ELISA

Kit Contents for Human Phospho-p53 (S46) DuoSet IC ELISA

Other Reagents Required

Preparation and Storage

Background: p53

Citations for Human Phospho-p53 (S46) DuoSet IC ELISA

Product Documents for Human Phospho-p53 (S46) DuoSet IC ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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