Human PDGF-DD Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DDD00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL), Saliva (50 µL), Human Milk (50 µL)
Sensitivity
3.51 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk)
Product Summary for Human PDGF-DD Quantikine ELISA Kit
The Quantikine Human PDGF-DD Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-DD in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human PDGF-DD and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-DD showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PDGF-DD.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PDGF-DD
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 176 | 510 | 986 | 198 | 559 | 1095 |
| Standard Deviation | 3.69 | 11.9 | 17.2 | 16.0 | 38.5 | 55.3 |
| CV% | 2.1 | 2.3 | 1.7 | 8.1 | 6.9 | 5.1 |
Recovery for Human PDGF-DD Quantikine ELISA Kit
The recovery of PDGF-DD spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 83-112 |
| Platelet-poor EDTA Plasma (n=4) | 93 | 83-109 |
| Platelet-poor Heparin Plasma (n=4) | 94 | 84-113 |
| Serum (n=4) | 101 | 86-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-DD were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PDGF-DD Quantikine ELISA Kit
Human PDGF-DD ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-DD
Platelet-derived growth factor-D (PDGF-D) is a cytokine that associates into disulfide-linked homodimers that bind to PDGF R beta and trigger signaling through PDGF R beta homodimers and PDGF R alpha/beta heterodimers. It is secreted as a latent homodimer which is activated by proteolysis to release a bioactive disulfide-linked dimer. PDGF-DD is widely expressed in embryonic and adult tissues. It functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant. PDGF-DD is upregulated in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers.
Long Name
Platelet-derived Growth Factor DD
Alternate Names
PDGFDD
Entrez Gene IDs
80310 (Human)
Gene Symbol
PDGFD
Additional PDGF-DD Products
Product Documents for Human PDGF-DD Quantikine ELISA Kit
Product Specific Notices for Human PDGF-DD Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.