Human PDGF-BB Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 4.5 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL) |
Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma |
Sensitivity: | 15 pg/mL |
Assay Range: | 31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) |
Human PDGF-BB Quantikine ELISA Kit Summary: | The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB. |
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Citations for Human PDGF-BB Quantikine ELISA Kit (53)
Citations are publications that use Bio-Techne products. Selected citations for Human PDGF-BB Quantikine ELISA Kit include:
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Species: Equine
Sample Types: Cell Culture Supernates
K Fukuda et al. (2022), Platelet lysate enhances equine skeletal muscle regeneration in a bupivacaine-induced muscle injury model Journal of equine veterinary science, 0(0):103892.
PMID: 35124154 -
Species: Human
Sample Types: Plasma
N Casagrande et al. (2021), In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel International Journal of Molecular Sciences, 22(6).
PMID: 33809553 -
Species: Human
Sample Types: Plasma
S Isozaki et al. (2021), Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators Mediators of Inflammation, 2021(0):5582943.
PMID: 33859537 -
Species: Human
Sample Types: Cell Culture Supernates
S Du et al. (2021), Anoikis resistant gastric cancer cells promote angiogenesis and peritoneal metastasis through C/EBPbeta-mediated PDGFB autocrine and paracrine signaling Oncogene, 0(0).
PMID: 34341514 -
Species: Human
Sample Types: Plasma
L Kobari et al. (2021), Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells Leukemia, 0(0).
PMID: 34556797 -
Species: Rat
Sample Types: Cell Culture Supernates
K Yamazaki et al. (2021), Mesenchymal Stem Cell Sheet Promotes Functional Recovery and Palliates Neuropathic Pain in a Subacute Spinal Cord Injury Model Stem Cells International, 2021(0):9964877.
PMID: 34306098 -
Species: Human
Sample Types: Serum
LH Lin et al. (2020), Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma Int J Mol Sci, 21(7).
PMID: 32235327 -
Species: Human
Sample Types: Serum
K Idemoto et al. (2020), Platelet-derived growth factor BB: A potential diagnostic blood biomarker for differentiating bipolar disorder from major depressive disorder Journal of psychiatric research, 134(0):48-56.
PMID: 33360224 -
Species: Human
Sample Types: Cell Culture Supernates
YY Liang et al. (2020), RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner Cell Death Dis, 11(10):855.
PMID: 33057010 -
Species: Equine
Sample Types: Cell Culture Supernates
K Fukuda et al. (2020), Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-&beta1 in equine platelet-rich plasma J. Vet. Med. Sci., 0(0).
PMID: 32814750 -
Species: Human
Sample Types: Cell Lysates
SC Notodihard et al. (2019), Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing Regen Ther, 10(0):1-9.
PMID: 30525065 -
Species: Human
Sample Types: Serum
TL Kuo et al. (2019), ?-catenin-activated autocrine PDGF/Src signaling is a therapeutic target in pancreatic cancer Theranostics, 9(2):324-336.
PMID: 30809277 -
Species: Human
Sample Types: Cell Culture Supernates
SI Sekine et al. (2019), Functional evaluation of PDGFB-variants in idiopathic basal ganglia calcification, using patient-derived iPS cells Sci Rep, 9(1):5698.
PMID: 30952898 -
Species: Rabbit
Sample Types: Platelet Rich Fibrin
MY Bai et al. (2018), Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels Sci Rep, 8(1):10642.
PMID: 30006555 -
Species: Human
Sample Types: Cell Culture Supernates
F Agostini et al. (2018), 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 13(9):e0203048.
PMID: 30188924 -
Species: Human
Sample Types: Scaffold Supernates
M Piran et al. (2018), In vitro fibroblast migration by sustained release of PDGF-BB loaded in chitosan nanoparticles incorporated in electrospun nanofibers for wound dressing applications Artif Cells Nanomed Biotechnol, 0(0):1-10.
PMID: 29361857 -
Species: Human
Sample Types: Cell Lysate
G Togliatto et al. (2018), PDGF-BB Carried by Endothelial Cell-derived Extracellular Vesicles Reduces Vascular Smooth Muscle Cell Apoptosis in Diabetes Diabetes, 0(0).
PMID: 29386225 -
Species: Human
Sample Types: Complex Sample Type
K Isobe et al. (2017), Platelet-rich fibrin prepared from stored whole-blood samples Int J Implant Dent, 3(1):6.
PMID: 28251561 -
Species: Human
Sample Types: Cell Lysates
M Bernardi et al. (2017), The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro J Transl Med, 15(1):90.
PMID: 28460641 -
Species: Equine
Sample Types: Plasma
G Hauschild et al. (2017), Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations BMC Vet. Res, 13(1):7.
PMID: 28056978 -
Species: Human
Sample Types: Plasma
Jan Pierce et al. (2017), Comparative analyses of industrial-scale human platelet lysate preparations. Transfusion, 0(0):1537-2995.
PMID: 28990195 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Unaltered Angiogenesis-Regulating Activities of Platelets in Mild Type 2 Diabetes Mellitus despite a Marked Platelet Hyperreactivity PLoS ONE, 11(9):e0162405.
PMID: 27612088 -
Species: Human
Sample Types: Platelet Rich Plasma (PRP)
Hideo Masuki (2016), Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF) Int J Implant Dent, 2(1):19.
PMID: 27747711 -
Species: Human
Sample Types: Cell Culture Supernates
D Dauwe et al. (2016), Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved J Am Heart Assoc, 5(4).
PMID: 27091182 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Stem Cells Int, 2016(0):6104780.
PMID: 27840648 -
Species: Porcine
Sample Types: Plasma
Samet Bayraktar (2016), Molecular and Microarray-based Analysis of Diversity among Resting and Osteogenically Induced Porcine Mesenchymal Stromal Cells of Several Tissue Origin J Tissue Eng Regen Med, 0(0).
PMID: 27966263 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling Sci Rep, 6(0):29029.
PMID: 27374240 -
Species: Human
Sample Types: CSF
Paul G et al. (2015), Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson's disease patients. J Clin Invest, 125(3):1339-46.
PMID: 25689258 -
Species: Human
Sample Types: Serum
Tan et al. (2015), The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients. BMC Med, 13(0):6.
PMID: 25583409 -
Species: Human
Sample Types: Whole Blood
Verstraete M et al. (2015), Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial. Br J Cancer, 112(8):1314-25.
PMID: 25867261 -
Species: Human
Sample Types: Plasma
Zehetner C et al. (2014), Systemic upregulation of PDGF-B in patients with neovascular AMD. Invest Ophthalmol Vis Sci, 55(1):337-44.
PMID: 24334449 -
Chen C et al. (2014), Regulation of T cell proliferation by JMJD6 and PDGF-BB during chronic hepatitis B infection. Sci Rep, 4(0):6359.
PMID: 25219359 -
Species: Human
Sample Types: Cell Culture Supernates
Liegl et al. (2014), Temsirolimus inhibits proliferation and migration in retinal pigment epithelial and endothelial cells via mTOR inhibition and decreases VEGF and PDGF expression. PLoS ONE, 9(2):e88203.
PMID: 24586308 -
Species: Human
Sample Types: Cell Culture Supernates
Ricard N et al. (2014), Increased pericyte coverage mediated by endothelial-derived fibroblast growth factor-2 and interleukin-6 is a source of smooth muscle-like cells in pulmonary hypertension. Circulation, 129(15):1586-97.
PMID: 24481949 -
Species: Human
Sample Types: Tissue Homogenates
Ho I et al. (2013), Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells, 31(1):146-55.
PMID: 23034897 -
Species: Human
Sample Types: Cell Lysates
Kinzebach S et al. (2013), Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol, 14(0):48.
PMID: 24168020 -
Species: Human
Sample Types: Cell Culture Supernates
Fujii T et al. (2008), Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression. Am. J. Physiol. Heart Circ. Physiol., 294(6):H2785-91.
PMID: 18441206 -
Species: Human
Sample Types: Plasma
Mathew P et al. (2008), Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer. Br. J. Cancer, 99(9):1426-32.
PMID: 18841158 -
Species: Porcine
Sample Types: Plasma
Kilian O et al. (2008), Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite. Biomaterials, 29(24):3429-37.
PMID: 18501961 -
Species: Mouse
Sample Types: Tissue Homogenates
Oku H et al. (2008), Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis. Eur. J. Pharmacol., 590(1):400-8.
PMID: 18598692 -
Species: Porcine
Sample Types: Cell Culture Supernates
Siegbahn A et al. (2007), TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC. Arterioscler. Thromb. Vasc. Biol., 28(1):135-41.
PMID: 17991872 -
Species: Human
Sample Types: Cell Culture Supernates
Neuhoff S et al. (2007), Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Exp. Hematol., 35(7):1119-31.
PMID: 17588481 -
Species: Human
Sample Types: Cell Culture Supernates
Dolloff NG et al. (2007), Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor. Cancer Res., 67(2):555-62.
PMID: 17234763 -
Species: Human
Sample Types: Plasma
Herrmann S et al. (2006), Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation. Bone, 39(5):1156-63.
PMID: 16863704 -
Species: Human
Sample Types: Plasma
Undas A et al. (2006), Effects of simvastatin on angiogenic growth factors released at the site of microvascular injury. Thromb. Haemost., 95(6):1045-7.
PMID: 16732391 -
Species: Human
Sample Types: Cell Culture Supernates
Lederle W et al. (2006), Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells. Am. J. Pathol., 169(5):1767-83.
PMID: 17071599 -
Species: Human
Sample Types: Cell Culture Supernates
Tejada ML et al. (2006), Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin. Cancer Res., 12(9):2676-88.
PMID: 16675559 -
Species: Human
Sample Types: Cell Culture Supernates
Nagineni CN et al. (2005), Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 203(1):35-43.
PMID: 15368539 -
Species: Human
Sample Types: Cell Culture Supernates
Numasaki M et al. (2005), IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 175(9):6177-89.
PMID: 16237115 -
Species: Bovine
Sample Types: Cell Culture Supernates
Dardik A et al. (2005), Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha. J. Vasc. Surg., 41(2):321-31.
PMID: 15768016 -
Species: Human
Sample Types: Cell Culture Supernates
Saika S et al. (2004), Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice. Lab. Invest., 84(10):1245-58.
PMID: 15273699 -
Species: Human
Sample Types: Cell Lysates
Zhou W et al. (2003), Dual regulation of proliferation and growth arrest in prostatic stromal cells by transforming growth factor-beta1. Endocrinology, 144(10):4280-4.
PMID: 12959966 -
Species: Porcine
Sample Types: Cell Lysates
Bracamonte MP et al. (2002), Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle. Am. J. Physiol. Heart Circ. Physiol., 283(3):H853-60.
PMID: 12181111
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Customer Reviews for Human PDGF-BB Quantikine ELISA Kit (2)
Customer Images
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 04/09/2018
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Sample Tested: PlasmaVerified Customer | Posted 10/20/2017
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Product Documents for Human PDGF-BB Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.