Human Neuropilin-1 Quantikine ELISA Kit

Name: Human Neuropilin-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DNRP10
Price: 705 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DNRP10

Citations (3)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Cell Lysates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (10 uL)
Sample Types:	Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
Sensitivity:	9.33 pg/mL
Assay Range:	78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Human Neuropilin-1 Quantikine ELISA Kit Summary:	The Quantikine Human Neuropilin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Neuropilin-1 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Neuropilin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Neuropilin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Neuropilin-1.

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Product Specifications for Human Neuropilin-1 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Neuropilin-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	336	967	1890	329	934	1833
Standard Deviation	17.9	70.4	54.7	19.8	48.4	90.4
CV%	5.3	7.3	2.9	6	5.2	4.9

Recovery

The recovery of human Neuropilin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	91	86-99
Cell Lysates (n=3)	101	94-108
Urine (n=4)	99	85-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Neuropilin-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Neuropilin-1 Quantikine ELISA Kit

	Human Neuropilin-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously known as neuropilin) and Neuropilin-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind distinct members of the class III secreted semaphorin subfamily that are implicated in repulsive axon guidance. Neuropilin extracellular domains contain two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. In the absence of ligands, neuropilins can form homo- and hetero-oligomers via their MAM domains. Expression in developing neurons of the central and peripheral nervous systems is somewhat overlapping but distinct. Npn-1 and Npn-2 are also receptors for VEGF165 on endothelial and tumor cells.

Item	Value
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Citations for Human Neuropilin-1 Quantikine ELISA Kit (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Neuropilin-1 Quantikine ELISA Kit include:

Species: Human
Sample Types: Aqueous Humor

Y Qin et al. (2022), ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization JCI Insight, 0(0).
PMID: 35653189
Species: Human
Sample Types: Serum

D Balalis et al. (2022), Serum Concentration of Selected Angiogenesis-Related Molecules Differs among Molecular Subtypes, Body Mass Index and Menopausal Status in Breast Cancer Patients Oncogene, 11(14).
PMID: 35887839
Species: Human
Sample Types: Cell Culture Supernates

D Dauwe et al. (2016), Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved J Am Heart Assoc, 5(4).
PMID: 27091182

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Product Documents for Human Neuropilin-1 Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.