Human IL-12 p70 Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HS120
Key Product Details
Assay Length
6.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)
Sensitivity
0.5 pg/mL
Assay Range
0.625-40 pg/mL (Cell Culture Supernates)
0.781-50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
0.781-50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-12 p70 Quantikine HS ELISA Kit
The Quantikine HS Human IL-12 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-12p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 5.32 | 13.0 | 25.0 | 2.86 | 8.49 | 29.6 |
| Standard Deviation | 0.24 | 0.71 | 1.89 | 0.5 | 0.85 | 2.43 |
| CV% | 4.5 | 5.5 | 7.6 | 17.5 | 10.0 | 8.2 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 5.26 | 18.6 | 35.8 | 2.86 | 9.25 | 36.4 |
| Standard Deviation | 0.26 | 0.77 | 0.90 | 0.36 | 1.39 | 2.76 |
| CV% | 4.9 | 4.1 | 2.5 | 12.6 | 15.0 | 7.6 |
Recovery for Human IL-12 p70 Quantikine HS ELISA Kit
The recovery of IL-12 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 95 | 85-107 |
| EDTA Plasma (n=5) | 102 | 83-117 |
| Heparin Plasma (n=5) | 97 | 86-111 |
| Serum (n=5) | 100 | 91-115 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-12 p70 Quantikine HS ELISA Kit
Human IL-12 p70 High Sensitivity ELISA Cell Culture Supernate Standard Curve
Human IL-12 p70 High Sensitivity ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12 p70
IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
Long Name
Interleukin 12 p70
Alternate Names
IL12 p70
Gene Symbol
IL12A
Additional IL-12 p70 Products
Product Documents for Human IL-12 p70 Quantikine HS ELISA Kit
Product Specific Notices for Human IL-12 p70 Quantikine HS ELISA Kit
For research use only
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