Human IGF-II/IGF2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DG200
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)
Sensitivity
5.41 pg/mL
Assay Range
12.5-800 pg/mL (Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human IGF-II/IGF2 Quantikine ELISA Kit
The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum, Serum-free Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 128 | 305 | 566 | 136 | 307 | 558 |
| Standard Deviation | 4.05 | 7.20 | 15.6 | 9.89 | 28.5 | 28.2 |
| CV% | 3.2 | 2.4 | 2.8 | 7.3 | 9.3 | 5.1 |
Recovery for Human IGF-II/IGF2 Quantikine ELISA Kit
The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum-free Cell Culture Media (n=4) | 95 | 82-104 |
| Urine (n=4) | 94 | 79-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IGF-II/IGF2 Quantikine ELISA Kit
Human IGF-II ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-II/IGF2
IGF-II/IGF2 (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) belongs to the insulin family of peptide growth factors. It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation. In human, IGF2 is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF2 levels drop after the perinatal period. IGF2 is produced by astrocytes, hepatocytes, osteoblasts, embryonic striated muscle cells plus Kupffer cells and Ito cells. IGF2 binds to IGF1R, Insulin R (IR)-type A, IGF1R:Insulin R-A hybrids, IGF2R, and IGF binding proteins 1-6. The first three receptors initiate downstream signaling events, the IGF2R sequesters local IGF2, and the six IGFBPs regulate IGF2 activity in various tissues.
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for Human IGF-II/IGF2 Quantikine ELISA Kit
Product Specific Notices for Human IGF-II/IGF2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.