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Human IFN-gamma Quantikine QuicKit ELISA
R&D Systems, Inc. a Bio-Techne Brand
Catalog # QK285
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Key Product Details
| Assay Length: | 80 minutes |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma |
| Sensitivity: | 2.56 pg/mL |
| Assay Range: | 31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) |
| Human IFN-gamma Quantikine QuicKit ELISA Summary: | The Quantikine™ QuicKit™ Human IFN-gamma Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma. |
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Human IFN-gamma QuicKit ELISA Spiked Recovery Competitor Comparison IFN-gamma is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 115% compared to 144% for the top competitor. Plasma recovery is 120% compared to 135% for the top competitor. Cell Culture Supernates recovery is 118% compared to 79% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices. |
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Human IFN-gamma QuicKit ELISA Spiked Linearity Competitor Comparison IFN-gamma is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-126% compared to 71%-85% for the top competitor. |
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Human IFN-gamma Standard Curve |
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Sample Tested: UrineVerified Customer | Posted 11/16/2022Quick, easy to follow protocol. Urine tested at 2-fold dilution and did not observe a matrix effect.
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