Human IFN-beta Quantikine QuicKit ELISA

Name: Human IFN-beta Quantikine QuicKit ELISA
Brand: R&D Systems
SKU: QK410

Catalog # QK410 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	80 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL)
Sensitivity:	2.88 pg/mL
Assay Range:	15.6 - 1,000 pg/mL (Cell Culture Supernates)
Human IFN-beta Quantikine QuicKit ELISA Summary:	The Quantikine™ QuicKit™ Human IFN-beta Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-beta levels in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and antibodies raised against the recombinant protein. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.

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Technical Data
Citations (2) / Reviews

Product Specifications for Human IFN-beta Quantikine QuicKit ELISA

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IFN-beta.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	101	613	121	669
Standard Deviation	3.82	30.1	6.48	33.3
CV%	3.8	4.9	5.4	5

Recovery

The recovery of human IFN-beta spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=)

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IFN-beta Quantikine QuicKit ELISA

	Human IFN-beta Standard Curve ELISA

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Item	Value
Long Name	Interferon beta
Entrez Gene IDs	3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names	Fibroblast interferon, IFB, IFBIFNB, IFF, IFN-beta, IFNB, IFNB1, IFNbeta, MGC96956, interferon beta, interferon, beta 1, fibroblast

Citations for Human IFN-beta Quantikine QuicKit ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human IFN-beta Quantikine QuicKit ELISA include:

Species: Human
Sample Types: Plasma

M Perego et al. (2022-06-21), Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns Oncogene, 2022-00(0).
PMID: 35726818
Species: Human
Sample Types: Serum

WJ Melvin et al. (2021-09-21), Coronavirus induces diabetic macrophage-mediated inflammation via SETDB2 Proceedings of the National Academy of Sciences of the United States of America, 2021-0118(38).
PMID: 34479991

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Product Documents for Human IFN-beta Quantikine QuicKit ELISA

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SDS

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