Human IFN-alpha 2 Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HSIFNA2
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
0.107 pg/mL
Assay Range
0.781-50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IFN-alpha 2 Quantikine HS ELISA Kit
The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IFN-alpha 2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to asses inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.38 | 15.1 | 32.3 | 2.65 | 15.5 | 29.7 |
| Standard Deviation | 0.082 | 0.686 | 1.36 | 0.150 | 0.965 | 1.69 |
| CV% | 3.4 | 4.5 | 4.2 | 5.7 | 6.2 | 5.7 |
Recovery for Human IFN-alpha 2 Quantikine HS ELISA Kit
The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 90-108 |
| EDTA Plasma (n=4) | 89 | 75-101 |
| Heparin Plasma (n=4) | 86 | 75-92 |
| Serum (n=4) | 86 | 75-99 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-alpha 2 Quantikine HS ELISA Kit
Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity
IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.
Human IFN-alpha 2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-alpha 2/IFNA2
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Long Name
Interferon alpha 2
Alternate Names
Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2
Gene Symbol
IFNA2
Additional IFN-alpha 2/IFNA2 Products
Product Documents for Human IFN-alpha 2 Quantikine HS ELISA Kit
Product Specific Notices for Human IFN-alpha 2 Quantikine HS ELISA Kit
For research use only
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