Human IFN-alpha 2 Quantikine HS ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
|Assay Length:||4.0 hours|
|Sample Type & Volume Required Per Well:||Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)|
|Sample Types:||Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma|
|Assay Range:||0.781 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)|
|Human IFN-alpha 2 Quantikine HS ELISA Kit Summary:||The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.|
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FAQs for Human IFN-alpha 2 Quantikine HS ELISA Kit
Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.