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Human IFN-alpha 2 Quantikine HS ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Catalog # HSIFNA2
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Key Product Details
| Assay Length: | 4.0 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma |
| Sensitivity: | 0.107 pg/mL |
| Assay Range: | 0.781 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) |
| Human IFN-alpha 2 Quantikine HS ELISA Kit Summary: | The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2. |
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Linearity
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Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum. |
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Human IFN-alpha 2 ELISA Standard Curve |
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FAQs for Human IFN-alpha 2 Quantikine HS ELISA Kit
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Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
