Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DHWAD0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.989 ng/mL
Assay Range
3.9-250 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
The Quantikine Human HMW Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HMW Adiponectin in serum and plasma. It contains NS0-expressed recombinant human HMW Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HMW Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HMW Adiponectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human high molecular weight Adiponectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human HMW Adiponectin in this assay. No medical histories were available for the donors
used in this study.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard deviation (ng/mL) |
| Male serum (n=12) | 4103 | 1686-6751 | 1892 |
| Female serum (n=24) | 7215 | 2595-11,882 | 2321 |
| Male EDTA plasma (n=12) | 3962 | 1539-6985 | 1926 |
| Female EDTA plasma (n=24) | 6842 | 2524-10,906 | 2215 |
| Male heparin plasma (n=12) | 3889 | 1590-6507 | 1807 |
| Female heparin plasma (n=24) | 6848 | 2445-11,948 | 2286 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 25.6 | 70.6 | 141 | 27.7 | 70.4 | 134 |
| Standard Deviation | 0.67 | 2.64 | 3.97 | 2.4 | 6.0 | 11.1 |
| CV% | 2.6 | 3.7 | 2.8 | 8.6 | 8.5 | 8.3 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of HMW Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
Human HMW Adiponectin/Acrp30 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Adiponectin (Acrp30) is secreted primarily by adipocytes and induces multiple paracrine and endocrine effects on metabolism and inflammation. Adiponectin promotes insulin sensitivity, decreased serum glucose and triglyceride levels, and increased serum glucagon levels. It promotes fatty acid uptake and oxidation, glucose uptake, and lactate production in skeletal muscle. Adiponectin promotes the development of M2 macrophages and promotes the C1qRp mediated clearance of apoptotic cell debris. Decreased plasma HMW Adiponectin levels are associated with upper body obesity, insulin resistance, reduced fatty acid oxidation, dyslipidemia, coronary artery disease, and atherogenesis. Adiponectin forms homotrimers, hexamers, and high molecular weight (HMW) complexes and signals through the receptors AdipoR1 and AdipoR2.
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
Product Specific Notices for Human HMW Adiponectin/Acrp30 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.