Human Free IGF-I/IGF-1 ELISA Kit

Name: Human Free IGF-I/IGF-1 ELISA Kit
Brand: R&D Systems
SKU: DFG100B
Rating: 5 (1 reviews)

Catalog # DFG100B | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (3)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	1.75 hours
Sample Type & Volume Required Per Well:	EDTA Plasma (50 uL)
Sensitivity:	0.006 ng/mL
Assay Range:	0.094 - 6 ng/mL (EDTA Plasma)
Human Free IGF-I/IGF-1 ELISA Kit Summary:	The Free IGF-I enzyme linked immunosorbent assay (ELISA) is a 1.75 hour solid-phase ELISA designed to measure human Free IGF-I in EDTA Plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor.

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Technical Data
Citations (3) / Reviews (1)

Product Specifications for Human Free IGF-I/IGF-1 ELISA Kit

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty-two separate assays to assess inter-assay precision

EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	32	32	32
Mean (ng/mL)	0.669	2.04	4.53	0.677	2.03	4.29
Standard Deviation	0.014	0.041	0.135	0.038	0.088	0.199
CV%	2.1	2	3	5.6	4.3	4.6

Linearity

Scientific Data Examples for Human Free IGF-I/IGF-1 ELISA Kit

	Human Free IGF-I ELISA Standard Curve
	Human Free IGF-I Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

IGF-1 (previously called somatomedin C) is a 7.6 kDa, 70 amino acid (aa) polypeptide with three internal disulfide bonds. The sequence of human IGF-1 is identical to that of bovine and porcine IGF-1, and it is 70% identical to human IGF-II. IGF-1 is a single-chain molecule with about 50% identity to the sequences of the A- and B-chains of human insulin.

While IGF-1 has very complex functions, it appears largely to mediate the actions of growth hormone. Thus, IGF-1 is important in prenatal development, growth to adulthood and metabolic control. It induces amino acid uptake, protein synthesis and glucose utilization. It is an important mitogen and regulator of the cell cycle and apoptosis. IGF-1 is produced primarily by hepatocytes, serving an endocrine function. It is also produced by many other cells, where it may act in an autocrine or paracrine manner. Serum levels of IGF-1 have been reported to increase from birth to puberty, followed by a slow decline through adulthood.

There are two receptors for the IGFs; type I IGF receptor, which signals through a tyrosine kinase, and type II IGF receptor, which is identical to the mannose-6-phosphate receptor and may not signal. The action of IGF on its receptors is very complex, with control by at least six IGF-binding proteins (IGFBP-1 through IGFBP-6). IGFBP-3 binds over 90% of the total IGF in serum in a complex of IGF, IGFBP and an acid-labile subunit. This ternary complex greatly stabilizes IGF in the circulation, changing the half life from minutes to hours. In addition, IGFBPs modulate the action of IGF on the membrane receptors. Adding to the complexity is a family of proteases that act on IGFBPs, modifying their affinity for IGF or completely eliminating the IGFBPs. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are referred to as the IGF axis. The IGF axis affects many primary physiological and pathological processes, including development, growth, metabolic regulation, tumorigenesis, atherosclerosis and angiogenesis.

Item	Value
Long Name	Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names	IBP1, IGF-1, IGF-I, IGF-IA, IGF-IB, IGF1, IGF1A, IGFI, MGF, Mechano growth factor, Somatomedin A, Somatomedin C, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, somatomedin-C

Citations for Human Free IGF-I/IGF-1 ELISA Kit (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Free IGF-I/IGF-1 ELISA Kit include:

Species: Bovine
Sample Types: Follicular Fluid

A García-Gue et al. (2018-03-01), Trio, a novel bovine high fecundity allele: III. Acquisition of dominance and ovulatory capacity at a smaller follicle size Biol. Reprod., 2018-00(0).
PMID: 29425314
Species: Porcine
Sample Types: Serum

(2016-12-09), Detection of Placental Proteomes at Different Uterine Positions in Large White and Meishan Gilts on Gestational Day 90 PLoS ONE, 2016-111(12):e0167799.
PMID: 27936087
Species: Human
Sample Types: Cell Culture Supernates

Yau S et al. (2014-11-05), IGFBP-2 inhibits adipogenesis and lipogenesis in human visceral, but not subcutaneous, adipocytes. Int J Obes (Lond), 2014-139(5):770-81.
PMID: 25370576

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Customer Reviews for Human Free IGF-I/IGF-1 ELISA Kit (1)

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Human Free IGF-I/IGF-1 ELISA Kit

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 02/25/2023

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Product Documents for Human Free IGF-I/IGF-1 ELISA Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Human Free IGF-I/IGF-1 ELISA Kit

Catalog # DFG100B | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Human Free IGF-I/IGF-1 ELISA Kit

Precision

Linearity

Scientific Data Examples for Human Free IGF-I/IGF-1 ELISA Kit

Preparation and Storage

Background: IGF-I/IGF-1

Citations for Human Free IGF-I/IGF-1 ELISA Kit (3)

Customer Reviews for Human Free IGF-I/IGF-1 ELISA Kit (1)

Human Free IGF-I/IGF-1 ELISA Kit

Product Documents for Human Free IGF-I/IGF-1 ELISA Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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