Human Follistatin Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DFN00
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Follicular Fluid (10 µL)
Sensitivity
83 pg/mL
Assay Range
250-16000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Follicular Fluid)
Product Summary for Human Follistatin Quantikine ELISA Kit
The Quantikine Human Follistatin Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human Follistatin (FS288, FS300, and FS315) in follicular fluid, cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Follistatin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human Follistatin. Results obtained using natural Follistatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Follistatin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Follistatin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1850 | 5363 | 10734 | 1645 | 4850 | 10256 |
| Standard Deviation | 59 | 136 | 181 | 139 | 312 | 584 |
| CV% | 3.2 | 2.5 | 1.7 | 8.4 | 6.4 | 5.7 |
EDTA Plasma, Follicular Fluid, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1996 | 6047 | 11999 | 1888 | 5604 | 11626 |
| Standard Deviation | 53 | 144 | 239 | 173 | 399 | 918 |
| CV% | 2.7 | 2.4 | 2.0 | 9.2 | 7.1 | 7.9 |
Recovery for Human Follistatin Quantikine ELISA Kit
The recovery of Follistatin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 91-107 |
| EDTA Plasma (n=5) | 96 | 87-102 |
| Serum (n=5) | 95 | 87-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Follistatin were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Follistatin Quantikine ELISA Kit
Human Follistatin ELISA Cell Culture Supernate Standard Curve
Human Follistatin ELISA Follicular Fluid/Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Follistatin
Follistatin (FST) is a secreted glycoprotein that binds and neutralizes several TGF-beta superfamily proteins including Activin, BMP-6, BMP-7, and GDF-8/Myostatin. It also binds Angiogenin and regulates hematopoietic stem cell adhesion to Fibronectin. Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3. Of three follistatin isoforms, the full-length mature follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative forms. The acidic tail is missing in the splice variant FST288, which shows highest affinity, and partial in the proteolytically produced FST303, which shows intermediate affinity. Genetic deletion of follistatin in mice, or expression of only the FST288 form, is lethal perinatally due to defects of lung, skin and musculoskeletal system. Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility.
Additional Follistatin Products
Product Documents for Human Follistatin Quantikine ELISA Kit
Product Specific Notices for Human Follistatin Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.