Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFL00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
5.1 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
The Quantikine® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Fas Ligand.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 92.1 | 240 | 587 | 98.6 | 244 | 576 |
| Standard Deviation | 3.31 | 7.51 | 18.2 | 4.41 | 8.70 | 25.7 |
| CV% | 3.6 | 3.1 | 3.1 | 4.5 | 3.6 | 4.5 |
Recovery for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
The recovery of human Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 93-103 |
| EDTA Plasma (n=4) | 103 | 94-111 |
| Heparin Plasma (n=4) | 96 | 92-101 |
| Serum (n=4) | 101 | 96-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Fas Ligand were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Human Fas Ligand/TNFSF6 Standard Curve
Human Fas Ligand/TNFSF6 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.
Alternate Names
CD178, CD95L, FASLG, TNFSF6
Gene Symbol
FASLG
Additional Fas Ligand/TNFSF6 Products
Product Documents for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Product Specific Notices for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.