Human Cytochrome c Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCTC0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Lysates (20 µL), Subcellular Fractions (100 µL)
Sensitivity
0.31 ng/mL
Assay Range
0.625-20 ng/mL (Cell Lysates, Subcellular Fractions)
Product Summary for Human Cytochrome c Quantikine ELISA Kit
The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human Cytochrome c
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates, Subcellular Fractions
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 3.64 | 7.31 | 10.7 | 3.54 | 6.74 | 10.2 |
| Standard Deviation | 0.15 | 0.30 | 0.42 | 0.18 | 0.34 | 0.51 |
| CV% | 4.1 | 4.1 | 3.9 | 5.1 | 5.0 | 5.0 |
Linearity
To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Cytochrome c Quantikine ELISA Kit
Human Cytochrome c ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cytochrome c
Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
Additional Cytochrome c Products
Product Documents for Human Cytochrome c Quantikine ELISA Kit
Product Specific Notices for Human Cytochrome c Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.