Human CXCL10/IP-10 Quantikine QuicKit ELISA Best Seller
R&D Systems, part of Bio-Techne | Catalog # QK266
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
4.1 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CXCL10/IP-10 Quantikine QuicKit ELISA
The Quantikine® QuicKit™ Human CXCL10/IP-10 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IP-10 levels in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human IP-10 and antibodies raised against the recombinant protein. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IP-10.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IP-10.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human IP-10 in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates:
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation |
| Serum (n=10) | 85.6 | 49.8-250 | 62.0 |
| EDTA plasma (n=10) | 99.4 | 60.4-205 | 51.8 |
| Heparin plasma (n=10) | 137 | 83.9-281 | 75.0 |
Cell Culture Supernates:
Human peripheral blood cells (PBMCs) (1 x 106
cells/mL) were cultured in RPMI supplemented
with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL
streptomycin sulfate. Cells were left unstimulated or stimulated with 10 μg/mL PHA for
5 days. Aliquots of the cell culture supernates were removed, assayed for levels of human IP-10,
and measured 12,555 pg/mL and 6570 pg/mL, respectively.
PBMCs were cultured in the medium as described above. Cells were left unstimulated or
stimulated with 1 μg/mL LPS and 40 ng/mL of recombinant human IFN-gamma for 24 hours. Aliquots
of the cell culture supernates were removed, assayed for levels of human IP-10, and measured
174 pg/mL and 958 pg/mL, respectively.
THP-1 human acute monocyte leukemia cells were left in RPMI supplemented with
10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin,
and 100 μg/mL streptomycin sulfate. Cells were left unstimulated or stimulated with
1 μg/mL of recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells
were incubated for an additional 18 hours. Aliquots of the cell culture supernates were
removed, assayed for levels of human IP-10, and measured 1172 pg/mL and 270,600 pg/mL,
respectively.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 96.7 | 521 | 95.3 | 515 |
| Standard Deviation | 1.12 | 10.6 | 10.8 | 42.1 |
| CV% | 1.2 | 2.0 | 11.3 | 8.2 |
Recovery for Human CXCL10/IP-10 Quantikine QuicKit ELISA
The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 119 | 110-129 |
| EDTA Plasma (n=2) | 86 | 78-95 |
| Heparin Plasma (n=2) | 104 | 92-118 |
| Serum (n=2) | 71 | 67-76 |
Linearity
Scientific Data Images for Human CXCL10/IP-10 Quantikine QuicKit ELISA
Human IP-10 ELISA Standard Curve
Human IP-10 QuicKit Spiked Recovery Competitor Comparison
IP-10 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 95% compared to 49% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human IP-10 QuicKit Spiked Linearity Competitor Comparison
IP-10 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-124% compared to 112%-183% for the top competitor.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Human CXCL10/IP-10 Quantikine QuicKit ELISA
Product Specific Notices for Human CXCL10/IP-10 Quantikine QuicKit ELISA
For research use only
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