Human Complement Factor D Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DFD00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.025 ng/mL
Assay Range
0.312-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Complement Factor D Quantikine ELISA Kit
The Quantikine Human Complement Factor D Immunoassay is a 3.5 hour sandwich ELISA designed to measure human Complement Factor D in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Complement Factor D and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Complement Factor D showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Complement Factor D.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Complement Factor D
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.860 | 3.12 | 8.13 | 0.831 | 3.04 | 7.73 |
| Standard Deviation | 0.047 | 0.183 | 0.552 | 0.074 | 0.211 | 0.506 |
| CV% | 6.4 | 5.9 | 5.8 | 9.0 | 6.9 | 6.6 |
Recovery for Human Complement Factor D Quantikine ELISA Kit
The recovery of Complement Factor D spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 97-117 |
| Urine (n=4) | 100 | 87-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Complement Factor D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Complement Factor D Quantikine ELISA Kit
Human Complement Factor D/Adipsin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Complement Factor D/Adipsin
Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.
Alternate Names
Adipsin, ADN, AMBP-1, CFD, PFD
Gene Symbol
CFD
Additional Complement Factor D/Adipsin Products
Product Documents for Human Complement Factor D Quantikine ELISA Kit
Product Specific Notices for Human Complement Factor D Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.