Human Caspase-1/ICE ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # DCA100
Key Product Details
Assay Length
3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL)
Sensitivity
1.24 pg/mL
Assay Range
6.25-400 pg/mL (Cell Culture Supernates)
Product Summary for Human Caspase-1/ICE Quantikine ELISA Kit
The Quantikine Human Caspase-1 Immunoassay is a 3 hour solid phase ELISA designed to measure Caspase-1 in cell culture supernates. It contains recombinant human Caspase-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Caspase-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Caspase-1. These results indicate that this kit can be used to determine relative mass values for natural human Caspase-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Caspase-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 41.0 | 77.7 | 228 | 40.4 | 75.2 | 219 |
| Standard Deviation | 2.49 | 4.62 | 11.11 | 3.8 | 6.9 | 18.1 |
| CV% | 6.1 | 5.9 | 4.9 | 9.4 | 9.2 | 8.3 |
Recovery for Human Caspase-1/ICE Quantikine ELISA Kit
The recovery of Caspase-1 spiked to three different levels throughout the range of the assay was evaluated
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 93 | 85-106 |
Linearity
To assess the linearity of the assay, cell culture supernate samples containing and/or spiked with high concentrations of Caspase-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Caspase-1/ICE Quantikine ELISA Kit
Human Caspase-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Caspase-1
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa and two 10 kDa subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine.
Additional Caspase-1 Products
Product Documents for Human Caspase-1/ICE Quantikine ELISA Kit
Product Specific Notices for Human Caspase-1/ICE Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.