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Human Carbonic Anhydrase IX Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Assay Length: | 4.5 hours |
| Sample Type & Volume Required Per Well: | Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL) |
| Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine |
| Sensitivity: | 4.39 pg/mL |
| Assay Range: | 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine) |
| Human Carbonic Anhydrase IX Quantikine ELISA Kit Summary: | The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9. |
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Human Carbonic Anhydrase IX/CA9 ELISA Standard Curve |
Citations for Human Carbonic Anhydrase IX Quantikine ELISA Kit (10)
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Citations are publications that use Bio-Techne products. Selected citations for Human Carbonic Anhydrase IX Quantikine ELISA Kit include:
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Species: Human
Sample Types: Seroma Fluid
N Oishi et al. (2020), Molecular profiling reveals a hypoxia signature in breast implant-associated anaplastic large cell lymphoma Haematologica, 0(0).
PMID: 32414854 -
Species: Human
Sample Types: Cell Culture Supernates
L Klameth et al. (2017), Small cell lung cancer: model of circulating tumor cell tumorospheres in chemoresistance Sci Rep, 7(1):5337.
PMID: 28706293 -
Species: Human
Sample Types: Urine
Shimizu Y et al. (2016), A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med, 14(1):31.
PMID: 26830497 -
Species: Human
Sample Types: Cell Lysates
(2016), DNA-linked Inhibitor Antibody Assay (DIANA) for sensitive and selective enzyme detection and inhibitor screening Nucleic Acids Res, 0(0).
PMID: 27679479 -
Species: Human
Sample Types: Urine
Goodison et al. (2012), A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS ONE, 7(10):e47469.
PMID: 23094052 -
Species: Human
Sample Types: Plasma
(2012), Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma. Br. J. Cancer, 107(7):1131-7.
PMID: 22918393 -
Species: Human
Sample Types: Plasma
de Groot JF et al. (2011), Myeloid Biomarkers Associated with Glioblastoma Response to Anti-VEGF Therapy with Aflibercept. Clin. Cancer Res., 17(14):4872-4881.
PMID: 21632852 -
Species: Human
Sample Types: Serum
Wind TC et al. (2011), Measuring carbonic anhydrase IX as a hypoxia biomarker: differences in concentrations in serum and plasma using a commercial enzyme-linked immunosorbent assay due to influences of metal ions. Ann. Clin. Biochem., 48(0):112-20.
PMID: 21367885 -
Species: Human
Sample Types: Plasma
Ilie M et al. (2010), High levels of carbonic anhydrase IX in tumour tissue and plasma are biomarkers of poor prognostic in patients with non-small cell lung cancer. Br. J. Cancer, 102(11):1627-35.
PMID: 20461082 -
Species: Human
Sample Types: Cell Culture Supernates
Tanaka N et al. (2008), Expression of carbonic anhydrase 9, a potential intrinsic marker of hypoxia, is associated with poor prognosis in oesophageal squamous cell carcinoma. Br. J. Cancer, 99(9):1468-75.
PMID: 18841153
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Customer Reviews for Human Carbonic Anhydrase IX Quantikine ELISA Kit (2)
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Sample Tested: Human blood serum,human breast implant seroma,human breast implant-associated anaplastic large cell lymphoma,Human PlasmaVerified Customer | Posted 12/02/2020Pubmed: 32414854
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Sample Tested: Human plasma samplesVerified Customer | Posted 02/21/2018Used 100 ul of Standard, control and plasma samples in duplicates.
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Product Documents for Human Carbonic Anhydrase IX Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
