Human Amphiregulin Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DAR00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)
Sensitivity
3.56 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Amphiregulin Quantikine ELISA Kit
The Quantikine Human Amphiregulin Immunoassay is a 4.5 hour solid
phase ELISA designed to measure human Amphiregulin in cell culture
supernates, cell lysates serum, plasma, saliva, and urine. It contains E. coli-derived
recombinant human Amphiregulin and has been shown to accurately quantitate the recombinant factor.
Results obtained with natural human Amphiregulin showed
linear curves that were parallel to the standard curves obtained using
the Quantikine kit standards. These results indicate that this kit can
be used to determine relative mass values for natural human Amphiregulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Recombinant and natural human Amphiregulin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 102 | 314 | 631 | 99.4 | 308 | 614 |
| Standard Deviation | 5.63 | 10.5 | 22.4 | 9.85 | 20.5 | 28.8 |
| CV% | 5.5 | 3.3 | 3.5 | 9.9 | 6.7 | 4.7 |
Recovery for Human Amphiregulin Quantikine ELISA Kit
The recovery of Amphiregulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-101 |
| EDTA Plasma (n=4) | 93 | 82-105 |
| Heparin Plasma (n=4) | 94 | 82-103 |
| Saliva (n=4) | 104 | 98-113 |
| Serum (n=4) | 95 | 85-103 |
| Urine (n=4) | 88 | 75-99 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Amphiregulin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Amphiregulin Quantikine ELISA Kit
Human Amphiregulin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Amphiregulin
Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.
Additional Amphiregulin Products
Product Documents for Human Amphiregulin Quantikine ELISA Kit
Product Specific Notices for Human Amphiregulin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.