Is ACE-1 the same as ACE?

Name: Human ACE Quantikine ELISA Kit
Brand: R&D Systems
SKU: DACE00

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Human ACE Quantikine ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DACE00

Citations (5)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (25 uL)
Sample Types:	Cell Culture Supernates, Serum, Heparin Plasma, Saliva
Sensitivity:	0.051 ng/mL
Assay Range:	0.78 - 50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva)
Human ACE Quantikine ELISA Kit Summary:	The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.

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Product Specifications for Human ACE Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	3.6	10.7	22.1	3.44	10.3	21.8
Standard Deviation	0.08	0.3	0.95	0.21	0.4	0.87
CV%	2.2	2.8	4.3	6.1	3.9	4

Serum, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (ng/mL)	3.57	10.9	22.5	3.77	11.7	24.4
Standard Deviation	0.12	0.43	0.9	0.29	0.6	1.2
CV%	3.4	3.9	4	7.7	5.1	4.9

Recovery

The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	98	89-106
Heparin Plasma (n=4)	100	94-106
Serum (n=4)	99	91-106

Linearity

To assess the linearity of the assay, samples containing high concentrations of ACE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human ACE Quantikine ELISA Kit

	Human ACE/CD143 ELISA Cell Culture Supernate/Saliva Standard Curve
	Human ACE/CD143 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ACE/CD143

ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. ACE exists in two isoforms. Somatic ACE comprises two highly similar protease domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome.

Item	Value
Long Name	Angiotensin I Converting Enzyme
Entrez Gene IDs	1636 (Human); 11421 (Mouse)
Alternate Names	ACE, ACE1angiotensin converting enzyme, somatic isoform, CD143, CD143 antigen, DCP, DCP1, DCP1angiotensin-converting enzyme, Dipeptidyl carboxypeptidase I, EC 3.2.1.-, EC 3.4.15.1, Kininase II, MGC26566, MVCD3, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, carboxycathepsin, dipeptidyl carboxypeptidase 1, peptidase P, testicular ECA

Citations for Human ACE Quantikine ELISA Kit (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human ACE Quantikine ELISA Kit include:

Species: Human
Sample Types: Plasma

PL Brito et al. (2022), Reduced blood pressure in sickle cell disease is associated with decreased angiotensin converting enzyme (ACE) activity and is not modulated by ACE inhibition PLoS ONE, 17(2):e0263424.
PMID: 35113975
Species: Human
Sample Types: Plasma

X Xu et al. (2019), RhoA-Rho associated kinase signaling leads to renin-angiotensin system imbalance and angiotensin converting enzyme 2 has a protective role in acute pulmonary embolism Thromb. Res., 176(0):85-94.
PMID: 30784777
Species: Human
Sample Types: Serum

S Yasar et al. (2018), Associations of Angiotensin Converting Enzyme-1 and Angiotensin II Blood Levels and Cognitive Function J. Alzheimers Dis., 0(0).
PMID: 29660936
Species: Human
Sample Types: Serum

Wang P et al. (2012), Circulating ACE is a predictor of weight loss maintenance not only in overweight and obese women, but also in men. Int J Obes (Lond), 36(12):1545-51.
PMID: 22270380
Species: Human
Sample Types: Plasma

Nielsen HM et al. (2007), Soluble adhesion molecules and angiotensin-converting enzyme in dementia. Neurobiol. Dis., 26(1):27-35.
PMID: 17270454

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Showing 1 - 5 of 5 citations Showing All

FAQs for Human ACE Quantikine ELISA Kit

Is ACE-1 the same as ACE?

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Product Documents for Human ACE Quantikine ELISA Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.