Rabbit HGF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-54125

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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

6.1 pg/mL (example only; lot dependent)

Assay Range

15.625 - 1000 pg/mL (example only; lot dependent)

View all HGF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rabbit

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Rabbit HGF ELISA Kit (Colorimetric)

Rabbit HGF ELISA Kit (Colorimetric) ELISA: Rabbit HGF ELISA Kit (Colorimetric) [NBP3-54125]

ELISA: Rabbit HGF ELISA Kit (Colorimetric) [NBP3-54125]

Kit Contents for Rabbit HGF ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HGF

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Gene Symbol

HGF

Additional HGF Products

Product Documents for Rabbit HGF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit HGF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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