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Mouse Growth Hormone ELISA Kit (Colorimetric)

Catalog # NBP3-08143 | Novus Biologicals a Bio-Techne Brand
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NBP3-08143

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10.00

Inter-Assay Precision (Precision between assays) %CV < 10.00

Recovery for Mouse Growth Hormone ELISA Kit (Colorimetric)

Recovery

89 - 104%

Linearity

Mouse Growth Hormone ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Growth Hormone ELISA Kit (Colorimetric)

ELISA: Mouse Growth Hormone ELISA Kit (Colorimetric) [NBP3-08143] - Standard Curve Reference

Kit Contents for Mouse Growth Hormone ELISA Kit (Colorimetric)

  • Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Growth Hormone

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Gene Symbol

GH1

Product Documents for Mouse Growth Hormone ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Growth Hormone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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