Mouse GP1BB ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-54234

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Key Product Details

Sample Type & Volume Required Per Well

Plasma (100 uL)

Sensitivity

14.7 pg/mL (example only; lot dependent)

Assay Range

31.25 - 2000 pg/mL (example only; lot dependent)

View all GP1BB ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Mouse GP1BB ELISA Kit (Colorimetric)

Mouse GP1BB ELISA Kit (Colorimetric) ELISA: Mouse GP1BB ELISA Kit (Colorimetric) [NBP3-54234]

ELISA: Mouse GP1BB ELISA Kit (Colorimetric) [NBP3-54234]

Kit Contents for Mouse GP1BB ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GP1BB

Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alphachain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor(VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, andGPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling throughphosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated withBernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIbbeta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arisingfrom a longer, unspliced transcript in endothelial cells has been described; however, the authenticity of this producthas been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb, expressed in nonhematopoietic tissuessuch as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signalwithin a separate gene (septin 5) located upstream of this gene. In the absence of polyadenylation from its ownimperfect site, the septin 5 gene uses the consensus polyA signal of this gene. (provided by RefSeq)

Long Name

Glycoprotein lb [Platelet] beta

Alternate Names

BDPLT1, CD42c, GPIb-Beta, GPIbb

Gene Symbol

GP1BB

Additional GP1BB Products

Product Documents for Mouse GP1BB ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse GP1BB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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