Bovine Folic acid ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59998

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.39 ng/mL

Assay Range

0.39 ng/mL - 100 ng/mL

View all Folic acid ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of bovine FA. No significant cross-reactivity or interference between bovine FA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Bovine Folic acid ELISA Kit (Colorimetric)

Recovery

92% (serum) and 98% (EDTA plasma)

Scientific Data Images for Bovine Folic acid ELISA Kit (Colorimetric)

ELISA: Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998]

ELISA: Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998]

ELISA: Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Bovine Folic acid ELISA Kit (Colorimetric)

Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998] -

Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998] - Standard Curve Reference

Kit Contents for Bovine Folic acid ELISA Kit (Colorimetric)

  • Assay plate (12 x 8 coated Microwells)
  • Biotin-conjugate (10 x concentrate)
  • Biotin-conjugate Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Folic acid

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for FA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated FA. A competitive inhibition reaction is launched between FA (Standards or samples) and biotin-conjugated FA with the pre-coated FA antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of FA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

Folate, Tetrahydrofolate, Tetrahydrofolic acid, THFA

Additional Folic acid Products

Product Documents for Bovine Folic acid ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bovine Folic acid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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