Human Folic acid ELISA Kit (Colorimetric)

Name: Human Folic acid ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-59966

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59966

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

6.25 pg/mL

Assay Range

12.5 pg/mL - 200 pg/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human Folic acid ELISA Kit (Colorimetric)

ELISA: Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966]

ELISA: Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] -

Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - Standard Curve Reference

Kit Contents for Human Folic acid ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Assay plate
HRP-conjugate (100 x concentrate)
HRP-conjugate Diluent
Instruction manual
Sample Diluent
Standard (10 x concentrate)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Folic acid

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated FA. The competitive inhibition reaction is launched between with HRP-conjugated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

Folate, Tetrahydrofolate, Tetrahydrofolic acid, THFA

Additional Folic acid Products

Product Documents for Human Folic acid ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - Human Folic acid ELISA Kit (Colorimetric)

COA

SDS

Download SDS

Product Specific Notices for Human Folic acid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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