Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31638
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)
Sensitivity
0.15 ng/mL (example only; lot dependent)
Assay Range
0.468 - 30 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Assay Length: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric)
ELISA: Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31638] -
ELISA: Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31638] - Standard Curve ReferenceKit Contents for Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric)
- Detection Solution A
- Detection Solution B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Fibulin 1
Fibulin 1 is a matricellular glycoprotein component of the elastic fiber core in connective tissues. It self-aggregates and additionally binds to several extracellular matrix and cellular proteins including Aggrecan, Versican, Fibronectin, ECM1, NOV/CCN3, and pro-HB-EGF. Fibulin 1c enhances ADAMTS-1 mediated cleavage of Aggrecan and Versican and can itself be cleaved by neutrophil elastase or MMP-13. It functions as a bridge between Fibrinogen and Fibrin, enabling Fibrin attachment to the platelet cell surface via Integrin alpha 2 beta 3. Fibulin 1 inhibits cardiomyocyte proliferation and Fibronectin-mediated cell adhesion and can promote bone formation.
Alternate Names
FBLN1, FIBL1
Gene Symbol
FBLN1
Additional Fibulin 1 Products
Product Documents for Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric)
Product Specific Notices for Human Fibulin 1 - Ready-To-Use ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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