Can it be used with the samples from sheep?

The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.

Can it be used with tissue homogenates?

This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.

Can this kit be used with rat serum/plasma?

Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.

Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used

This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.

Is this assay for use in rat plasma? The species is not listed.

The kit should work for any species as the target should be the same across all species.

Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Name: Epinephrine/Norepinephrine ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1877

Novus Biologicals, part of Bio-Techne | Catalog # KA1877

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Plasma, Urine (Adrenaline ELISA : 100 uL (extracted)
Noradrenaline ELISA : 20 uL (extracted))

Sensitivity

Adrenaline: 0.9 ng/mL Urine, 10 pg/mL Plasma
Noradrenaline: 1.7 ng/mL Urine, 36 pg/mL Plasma

Assay Range

Adrenaline: 0.7 to 200 ng/mL in Urine; 18 to 6667 pg/mL in Plasma, Noradrenaline: 2.5 to 1000 ng/mL in Urine; 93 to 33333 pg/mL in Plasma

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Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Description

Quality control test: Standard curve. Epinephrine/Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Epinephrine and Norepinephrine in plasma and urine. This product contains two assay plates, for Epinephrine and Normetanephrine separately.

Precision

Intra-Assay Precision (Precision within an assay) Epinephrine: 12.57%, Norepinephrine: 12.95%

Inter-Assay Precision (Precision between assays) Epinephrine: 15.6%, Norepinephrine: 11.75%

Recovery for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Recovery

Epinephrine: 107% in Urine, 92% in Plasma Norepinephrine: 109% in Urine, 97% in Plasma

Scientific Data Images for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Epinephrine/Norepinephrine ELISA Kit [KA1877] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Acylation Buffer: Ready to use. Buffer with light alkaline pH for the acylation.
Acylation Reagent: Ready to use. Acylation reagent in DMF and DMSO.
Adhesive Foil: Ready to use. Adhesive foils in a resealable pouch.
Adjustment Buffer: Ready to use, TRIS buffer.
Adrenaline Antiserum: Ready to use, rabbit anti-adrenaline antibody, blue coloured.
Adrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in a resealabel blue pouch with desiccant.
Assay Buffer: Ready to use, 1 M hydrochloric acid and a non-mercury preservative.
Coenzyme: Ready to use, S-adenosyl-L-methionine.
Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins, conjugated with peroxidase.
Enzyme: Lyophilized Catechol-O-methyltransferase.
Extraction Buffer: Ready to use, buffer containing carbonate.
Extraction Plate: Ready to use, coated with boronate affinity gel in a resealabel pouch.
Hazards identification: H225 Highly flammable liquid and vapour. H360 May damage fertility or the unborn child. H319 Causes serious eye irritation
Hydrochloric Acid: Ready to use, 0.025 M hydrochloric acid, yellow coloured.
Noradrenaline Antiserum: Ready to use, rabbit anti-noradrenaline antibody, yellow coloured.
Noradrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in a resealable yellow pouch with desiccant.
Stop Solution: Ready to use, 0.25 M sulfuric acid. Hazards identification: H290 May be corrosive to metals. H314 Causes severe skin burns and eye damage.
Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide.
Wash Buffer Concentrate: 50x concentrated buffer with a non-ionic detergent and physiological pH.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Epinephrine/Norepinephrine

Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Alternate Names

adrenaline, Arterenol, l-Adrenaline, L-epinephrine, Levarterenol, Levoepinephrine, L-Noradrenaline, L-Norepinephrine, noradrenaline

Additional Epinephrine/Norepinephrine Products

Product Documents for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download KA1877 Manual

COA

Product Specific Notices for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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