Skip to main content

Multi-Species dsRNA ELISA Kit (Colorimetric)

Catalog # NBP3-11367 | Novus Biologicals a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-11367-200Tests
NBP3-11367-500Tests

Key Product Details

Sensitivity

10 ng/ml

Assay Range

3 - 300 ng/ml (0.3 - 30 ng/well)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Multi-Species

Specificity

We recommend using the kit to detect viral dsRNAs or large natural or synthetic dsRNAs of non-viral origin in nucleic acid extracts, as well as to detect the presence of undesired dsRNA molecules in artificially synthesized (m)RNA preparations. Serial dilutions of the Poly(I:C) dsRNA standard (included in the kit) can be used as a positive control.
For the exact detection protocol we refer to the kit manual that can be downloaded from our website.

Caution: The Poly(I:C) dsRNA positive control included in this sandwich ELISA kit is not intended to be used as a quantitative standard for other dsRNA preparations. Low molecular weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of our Poly(I:C) LMW is 0.2 kb to 1 kb. The anti dsRNA antibodies K1 and/or K2 used in this kit may exhibit a different degree of reactivity with different dsRNAs obtained from synthetic or natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out for the OD450.

Description

This assay works on the sandwich-ELISA principle and uses the J2 (IgG2a) mouse monoclonal antibody to dsRNA as a catcher antibody. The monoclonal antibody K2 (IgM) is used as the detector antibody. Based on the use of two double-stranded RNA (dsRNA)-specific monoclonal antibodies the dsRNA Detection Kit allows sensitive and selective detection of dsRNA molecules (larger than 30-40 bp), independent of their nucleotide composition and sequence. The detection is highly specific: dsRNA can be detected in nucleic acid extracts in the presence of 1.000-10.000-fold excess of other nucleic acids.

Source
The two dsRNA antibodies used in this kit were produced as follows: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clones J2 and K2.

Precision

Intra-Assay Precision (Precision within an assay) 5 - 10%

Inter-Assay Precision (Precision between assays) 5 - 10%

Kit Contents for Multi-Species dsRNA ELISA Kit (Colorimetric)

  • 1 vial of coating antibody (store at -20C)
  • 1 vial of dsRNA-specific detecting antibody (in RPMI + 5% FBS. Store at +4C or preferably aliquoted at -20C)
  • 1 vial of HRP-conjugated F(ab’)2 Fragment of goat-anti mouse secondary antibody (store at +4C or aliquoted at -20C)
  • 1 vial of Poly(I:C) dsRNA as positive control (store at -20C)
  • 1 vial of TMB substrate solution (Store at +4C. Keep in dark)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: dsRNA

Based on the use of two double-stranded RNA (dsRNA)-specific monoclonal antibodies the dsRNA Detection Kit allows sensitive and selective detection of dsRNA molecules (larger than 30-40 bp), independent of their nucleotide composition and sequence. The detection is highly specific: dsRNA can be detected in nucleic acid extracts in the presence of 1.000-10.000-fold excess of other nucleic acids. This assay works on the sandwich-ELISA principle and uses the J2 (IgG2a) mouse monoclonal antibody to dsRNA as a catcher antibody. The monoclonal antibody K2 (IgM) is used as the detector antibody.

Alternate Names

Double-stranded RNA

Product Documents for Multi-Species dsRNA ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Multi-Species dsRNA ELISA Kit (Colorimetric)

This ds-RNA antibody was manufactured by Scicon.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Loading...