DHEA ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07909
Key Product Details
Sample Type & Volume Required Per Well
Culture supernatants, plasma, serum, saliva, and urine (100 ul)
Sensitivity
2.9 pg/ml
Assay Range
12.21 - 50000 pg/ml
Product Specifications
Assay Type
Competitive ELISA
Kit Type
ELISA Kit (Colorimetric)
Specificity
Cross reactivities for a number of related steroid compounds:
DHEA sulfate 30%
Androstenedione 0.72%
Androsterone 0.29%
Pregnenolone 0.28%
Testosterone 0.10%
Progesterone 0.06%
Estradiol 0.04%
20alpha-hydroxycholesterol <0.02%
Androsterone sulphate <0.02%
DHEA sodium sulfate <0.02%
17alpha-hydroxyepipregnanolone <0.02%
19-hydroxyandrostendione <0.02%
17alpha-hydroxyprogesterone <0.02%
Cortisol <0.02%
DHEA sulfate 30%
Androstenedione 0.72%
Androsterone 0.29%
Pregnenolone 0.28%
Testosterone 0.10%
Progesterone 0.06%
Estradiol 0.04%
20alpha-hydroxycholesterol <0.02%
Androsterone sulphate <0.02%
DHEA sodium sulfate <0.02%
17alpha-hydroxyepipregnanolone <0.02%
19-hydroxyandrostendione <0.02%
17alpha-hydroxyprogesterone <0.02%
Cortisol <0.02%
Precision
Intra-Assay Precision (Precision within an assay) CV% < 10%
Inter-Assay Precision (Precision between assays) CV% < 10%
Recovery for DHEA ELISA Kit (Colorimetric)
Recovery
Tissue Culture Media: 95.2%
Human Saliva: 109.8%
Human Urine: 108.4%
Human Serum: 98.5%
Human heparin Plasma: 100.4%
Human Saliva: 109.8%
Human Urine: 108.4%
Human Serum: 98.5%
Human heparin Plasma: 100.4%
Scientific Data Images for DHEA ELISA Kit (Colorimetric)
Non-species specific DHEA ELISA Kit (Colorimetric) [NBP3-07909] - Standard curve
Kit Contents for DHEA ELISA Kit (Colorimetric)
- Assay Buffer Concentrate
- DHEA Assay Layout Sheet
- DHEA ELISA Antibody
- DHEA ELISA Conjugate
- DHEA Standard
- Goat anti-Rabbit IgG Plates
- Plate Sealer
- pNpp Substrate
- Stop Solution
- Wash Buffer Concentrate
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Product Documents for DHEA ELISA Kit (Colorimetric)
Product Specific Notices for DHEA ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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