Human Cytomegalovirus Antibody (IgG) ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59972
Key Product Details
Sample Type & Volume Required Per Well
Serum (50 - 100 uL)
Product Specifications
Assay Type
Indirect ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human anti-cytomegalovirus(CMV) antibody (IgG). No significant cross-reactivity or interference between human anti-cytomegalovirus(CMV) antibody (IgG) and analogues was observed.
Description
Assay Length: 1-5 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 15
Inter-Assay Precision (Precision between assays) %CV < 15
Kit Contents for Human Cytomegalovirus Antibody (IgG) ELISA Kit (Colorimetric)
- Adhesive Strip (For 96 wells)
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Sample Diluent
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Cytomegalovirus Antibody (IgG)
Cytomegalovirus is a common virus, infecting ~60% of adults in developed countries and over 90% in developing countries, as indicated by presence of specific IgG antibodies (2-4). Although a majority of people infected remain asymptomatic, immunocompromised people, organ transplant recipients, and fetuses and newborns are more susceptible to HCMV-associated diseases (1-4). Annually, up to 2% of newborns are born with HCMV, making it the most common congenital infection (2-4). Cytomegalovirus is known to cause sensorineural hearing loss (SNHL), mental retardation, chorioretinitis, skin lesions, as well as end organ disease (3,5). There are a few CMV therapeutics approved by FDA including valganciclovir and letermovir; however, despite some benefits, there is the need for new antivirals and combination therapies (5). Potential new treatment options include monoclonal antibodies and sirtuins (5).
References
1. Connolly SA, Jardetzky TS, Longnecker R. The structural basis of herpesvirus entry. Nat Rev Microbiol. 2021;19(2):110-121. https://doi.org/10.1038/s41579-020-00448-w
2. Griffiths P, Reeves M. Pathogenesis of human cytomegalovirus in the immunocompromised host. Nat Rev Microbiol. 2021;19(12):759-773. https://doi.org/10.1038/s41579-021-00582-z
3. Krstanovic F, Britt WJ, Jonjic S, Brizic I. Cytomegalovirus Infection and Inflammation in Developing Brain. Viruses. 2021;13(6):1078. Published 2021 Jun 4. https://doi.org/10.3390/v13061078
4. Griffiths P, Baraniak I, Reeves M. The pathogenesis of human cytomegalovirus. J Pathol. 2015;235(2):288-297. https://doi.org/10.1002/path.4437
5. Acosta E, Bowlin T, Brooks J, et al. Advances in the Development of Therapeutics for Cytomegalovirus Infections. J Infect Dis. 2020;221(Suppl 1):S32-S44. https://doi.org/10.1093/infdis/jiz493
Additional Cytomegalovirus Antibody (IgG) Products
Product Documents for Human Cytomegalovirus Antibody (IgG) ELISA Kit (Colorimetric)
Product Specific Notices for Human Cytomegalovirus Antibody (IgG) ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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