Mouse CTX-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11802

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all CTX-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.45

Inter-Assay Precision (Precision between assays) %CV < 5.73

Recovery for Mouse CTX-1 ELISA Kit (Colorimetric)

Recovery

86 - 108%

Linearity

Mouse Cross-linked C-Telopeptide of Type I Collagen ELISA Kit (Colorimetric)
Mouse CTX-1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse CTX-1 ELISA Kit (Colorimetric)

Mouse CTX-1 ELISA Kit (Colorimetric)

ELISA: Mouse CTX-1 ELISA Kit (Colorimetric) [NBP3-11802] -

ELISA: Mouse CTX-1 ELISA Kit (Colorimetric) [NBP3-11802] - Standard Curve Reference

Kit Contents for Mouse CTX-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Antibody Diluent
  • Concentrated Biotinylated Detection Antibody
  • Concentrated HRP Conjugate
  • Concentrated Wash Buffer
  • HRP Conjugate Diluent
  • Micro ELISA Plate
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CTX-1

CTX-1, also referred to as either carboxy-terminal cross-linked telopeptide of type 1 collagen or C-terminal end of the telopeptide of type I collagen, is a marker of osteoporosis and bone resorption (1,2). CTX-1 is a common bone turnover marker (BTM) that can easily be measured in the plasma, serum, or urine (1-3). Bone turnover refers to the removal of old bones by resorption followed by generation of new bones (3). CTX-1 is released from intact collagen type 1 by cathepsin K cleavage and can be detected by an antibody against the eight amino acid sequence EKAHDGGR (2,4). Levels of CTX-1 show circadian rhythm variabilities altered by food intake, which makes timing of sample collection an important consideration and is typically done in a fasted state (1,3). Studies show that increased levels of CTX-1 along with bone-specific alkaline phosphatase (BSALP) is associated with increased risk for bone fractures and osteoporosis (3). The most common treatment for osteoporosis is bisphosphonates, which are classified as anti-resorptive drugs that slow the removal of bone (1,3). Another treatment is the Denosumab, a monoclonal antibody for receptor activator of NFkappaB ligand, which shows decreased levels of CTX-1 and other markers within the first 24-hours of treatment (1-3). Conversely, anabolic therapies add bone by focusing on the osteoclast and osteoblast populations. Romosozumab is one specific anabolic drug that showed quick decrease in CTX-1 levels following treatment (2,3).

References

1. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

2. Szulc P. (2018). Bone turnover: Biology and assessment tools. Best Practice & Research. Clinical Endocrinology & Metabolism. https://doi.org/10.1016/j.beem.2018.05.003

3. Park, S. Y., Ahn, S. H., Yoo, J. I., Chung, Y. J., Jeon, Y. K., Yoon, B. H., Kim, H. Y., Lee, S. H., Lee, J., & Hong, S. (2019). Position Statement on the Use of Bone Turnover Markers for Osteoporosis Treatment. Journal of Bone Metabolism. https://doi.org/10.11005/jbm.2019.26.4.213

4. Cremers, C., Garnero, P., Seibel, M. J. (2008). Chapter 87- Biochemical Markers of Bone Metabolism. Principles of Bone Biology (Third Edition). https://doi.org/10.1016/B978-0-12-373884-4.00020-3.

Alternate Names

Collagen Type 1 C-Telopeptide, Cross Linked C-telopeptide of Type I Collagen, CTX, CTXI

Additional CTX-1 Products

Product Documents for Mouse CTX-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse CTX-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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