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cAMP Parameter Assay Kit

Multi-species cAMP ELISA Cell Lysis Buffer 5 (1X) Standard Curve
(3)
Multi-species cAMP ELISA Calibrator Diluent RD5-55 Standard Curve
Multi-species cAMP ELISA Linearity
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KGE002B
PKGE002B
SKGE002B

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Key Product Details

Assay Length: 3.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (10 uL)
Sensitivity: 8.57 pmol/mL
Assay Range: 3.75 - 240 pmol/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
cAMP Parameter Assay Kit Summary: The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.

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FAQs

  • The cAMP Parameter Assay Kit, Catalog # KGE002B, provides  a cell lysate preparation protocol. Can the cell freezing times be varied when using this protocol? 

    Cell lysates were prepared using the lysate preparation protocol as described. While our scientists have not tested alternate preparation protocols, alteration of the freezing time for cells (including overnight freezing) and using more than two freeze-thaws is not expected to alter the assay results. To be safe, user can determine this with some preliminary testing.

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.