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Human BPDE ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70012

Novus Biologicals, part of Bio-Techne
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NBP2-70012

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

9.38 ng/mL

Assay Range

15.63 - 1000 ng/mL

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human BPDE in samples. No significant cross-reactivity or interference between Human BPDE and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.54%

Inter-Assay Precision (Precision between assays) CV% < 5.17%

Recovery for Human BPDE ELISA Kit (Colorimetric)

Recovery

89-108%

Linearity

Human BPDE ELISA Kit (Colorimetric)

Scientific Data Images for Human BPDE ELISA Kit (Colorimetric)

Human BPDE ELISA Kit (Colorimetric)

ELISA: Human BPDE ELISA Kit (Colorimetric) [NBP2-70012] -

ELISA: Human BPDE ELISA Kit (Colorimetric) [NBP2-70012] - Standard Curve Reference

Kit Contents for Human BPDE ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BPDE

Benzo[a]pyrene is a polycyclic aromatic hydrocarbon found in cigarette smoke, heavily polluted air, and smoked foods. A diol epoxide derivative of benzo[a]pyrene, 7b, 8a-dihydroxy-9a,10a-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I) has been implicated as a key benzo[a]pyrene metabolite involved in covalent modification of DNA.

Long Name

Benzo[A] Pyrene-Diol Epoxide

Alternate Names

Benzo Pyrene Dihydrodiol Epoxide

Additional BPDE Products

Product Documents for Human BPDE ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human BPDE ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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