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Rat beta Galactosidase ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49751

Novus Biologicals, part of Bio-Techne
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NBP3-49751

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rat

Specificity

This kit recognizes Rat beta Galactosidase in samples. No significant cross-reactivity or interference between Rat beta Galactosidase and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Rat beta Galactosidase ELISA Kit (Chemiluminescence)

Recovery

80 - 120%

Linearity

Rat beta Galactosidase ELISA Kit (Chemiluminescence)

Scientific Data Images for Rat beta Galactosidase ELISA Kit (Chemiluminescence)

Rat beta Galactosidase ELISA Kit (Chemiluminescence)

ELISA: Rat beta Galactosidase ELISA Kit (Chemiluminescence) [NBP3-49751]

Standard Curve Reference.

Kit Contents for Rat beta Galactosidase ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate(100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: beta Galactosidase

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Alternate Names

EC 3.2.1.23; b-gal

Additional beta Galactosidase Products

Product Documents for Rat beta Galactosidase ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat beta Galactosidase ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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