Human ASAH2/N-acylsphingosine Amidohydrolase-2 - Ready-To-Use ELISA Kit (Colorimetric) New
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-54069
Key Product Details
Sample Type & Volume Required Per Well
Tissue homogenates and other biological fluids (100 uL)
Sensitivity
0.225 ng/mL (example only; lot dependent)
Assay Range
0.625 - 40 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Assay Length: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images
![Human ASAH2/N-acylsphingosine Amidohydrolase-2 - Ready-To-Use ELISA Kit (Colorimetric) ELISA: Human ASAH2/N-acylsphingosine Amidohydrolase-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54069]](https://resources.bio-techne.com/images/products/elisa/nbp3-54069_-elisa-639197507790501772.png "ELISA: Human ASAH2/N-acylsphingosine Amidohydrolase-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54069]")
ELISA: Human ASAH2/N-acylsphingosine Amidohydrolase-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54069]
Kit Contents
- Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual
Preparation and Storage
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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