Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-54068

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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.122 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

View all ASAH1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric)

Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric) ELISA: Mouse ASAH1  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54068]

ELISA: Mouse ASAH1  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-54068]

Kit Contents for Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ASAH1

ASAH1 encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Acid ceramidase

Alternate Names

AC, ACDase, Acid CDase, ASAH, EC 3.5.1.-, EC 3.5.1.23, HSD-33, HSD33

Gene Symbol

ASAH1

Additional ASAH1 Products

Product Documents for Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse ASAH1 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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