Angiotensin I ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62134

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NBP2-62134
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Key Product Details

Sample Type & Volume Required Per Well

Plasma,serum (50 uL)

Sensitivity

4.3 pg/ml

Assay Range

3.9-10,000 pg/ml

View all Angiotensin I ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

Crossreactivity: Angiotensin I (100%), Angiotensin A (0.182%), Angiotensin II (0.083%), Angiotensin (1-12) (0.025%). Unaffected by Angiotensin (1-9), Angiotensin (1-7), Angiotensin III, Angiotensin IV, LVV-hemorphin 7, Bradykinin.

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 10.0% Medium Concentration %CV - 7.3% High Concentration %CV - 8.3%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 7.6% Medium Concentration %CV - 10.0% High Concentration %CV - 7.6%

Recovery for Angiotensin I ELISA Kit (Colorimetric)

Recovery

Low Concentration %CV - 76.0%
Medium - 90.0%
High - 66.0%

Scientific Data Images for Angiotensin I ELISA Kit (Colorimetric)

ELISA: Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134]

ELISA: Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134]

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - Pooled human plasma was spiked with 500pg/ml of Angiotensin I, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was processed identically as a background control.
ELISA: Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134]

ELISA: Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134]

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - A typical standard curve for Angiotensin I ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Angiotensin I ELISA Kit (Colorimetric)

  • Antibody
  • Assay Buffer 16
  • Conjugate
  • Microtiter Plate
  • Standard
  • Stop Solution 2
  • Streptavidin-HRP
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Angiotensin I

The protein encoded by the Angiotensin I gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Alternate Names

alpha-1 antiproteinase, antitrypsin, angiotensin II, angiotensinogen, angiotensinogen (serpin peptidase inhibitor, clade A, member 8), ANHU, FLJ92595, FLJ97926, pre-angiotensinogen, serine (or cysteine) proteinase inhibitor, Serpin A8, SERPINA8angiotensin I

Gene Symbol

AGT

Additional Angiotensin I Products

Product Documents for Angiotensin I ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiotensin I ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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