Skip to main content

Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-66384

Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-66384

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human ACC in samples. No significant cross-reactivity or interference between Human ACC and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.05%

Inter-Assay Precision (Precision between assays) CV% < 5.3%

Recovery for Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

Recovery

85-106%

Linearity

Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

Scientific Data Images for Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

ELISA: Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric) [NBP2-66384] -

ELISA: Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric) [NBP2-66384] - Standard Curve Reference

Kit Contents for Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Acetyl-CoA Carboxylase alpha/ACACA

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Gene Symbol

ACACA

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents for Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Acetyl-CoA Carboxylase alpha/ACACA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Loading...
Loading...
Loading...