6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61286

Novus Biologicals, part of Bio-Techne
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NBP2-61286
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Key Product Details

Sample Type & Volume Required Per Well

Serum, saliva, and urine from any species (100 uL)

Sensitivity

1.40 pg/ml

Assay Range

3.2 - 50,000 pg/ml

View all 6-Keto-Prostaglandin F1A ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Precision

Intra-Assay Precision (Precision within an assay) Mean 6.9%

Inter-Assay Precision (Precision between assays) Mean 6.3%

Recovery for 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

Recovery

Tissue Culture Media - 106.7%
Human Saliva - 110.7%
Human Urine - 108.7%
Human Serum - 108.4%

Scientific Data Images for 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

ELISA: 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric) [NBP2-61286] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

  • Antibody
  • Assay buffer
  • Conjugate
  • DxS IgG Microtiter plate
  • Standard, pNpp Substrate
  • Stop solution
  • Wash buffer concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: 6-Keto-Prostaglandin F1A

6-keto-PGF1a is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1a metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1a. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.

Additional 6-Keto-Prostaglandin F1A Products

Product Documents for 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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