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3-Nitrotyrosine ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-66363

Catalog #
Size / Price

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)


0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type


Kit Type

ELISA Kit (Colorimetric)


This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed.


Intra-Assay Precision (Precision within an assay) CV% < 5.43%

Inter-Assay Precision (Precision between assays) CV% < 5.22%

Recovery for 3-Nitrotyrosine ELISA Kit (Colorimetric)




3-Nitrotyrosine ELISA Kit (Colorimetric)

Scientific Data Images for 3-Nitrotyrosine ELISA Kit (Colorimetric)

3-Nitrotyrosine ELISA Kit (Colorimetric)

ELISA: 3-Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-66363] -

ELISA: 3-Nitrotyrosine ELISA Kit (Colorimetric) [NBP2-66363] - Standard Curve Reference

Kit Contents for 3-Nitrotyrosine ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage


The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 3-Nitrotyrosine

Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure.

Additional 3-Nitrotyrosine Products

Product Documents for 3-Nitrotyrosine ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 3-Nitrotyrosine ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.