Human 26S Proteasome ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66362

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

View all 26S Proteasome ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human 26S-PSM in samples. No significant cross-reactivity or interference between Human 265-PSM and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.8%

Inter-Assay Precision (Precision between assays) CV% < 10.8%

Recovery for Human 26S Proteasome ELISA Kit (Chemiluminescence)

Recovery

88-108%

Linearity

Human 26S Proteasome ELISA Kit (Chemiluminescence)

Scientific Data Images for Human 26S Proteasome ELISA Kit (Chemiluminescence)

Human 26S Proteasome ELISA Kit (Chemiluminescence)

ELISA: Human 26S Proteasome ELISA Kit (Chemiluminescence) [NBP2-66362] -

ELISA: Human 26S Proteasome ELISA Kit (Chemiluminescence) [NBP2-66362] - Standard Curve Reference

Kit Contents for Human 26S Proteasome ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 26S Proteasome

The 26S Proteasome is the major non-lysosomal protease in eukaryotic cells and is responsible for the degradation of ubiquitinated substrates and misfolded proteins. It is composed of two subcomplexes: the 20S Proteasome core particle and the 19S Proteasome regulatory particle. The 20S Proteasome facilitates proteolytic cleavage of protein substrates and is composed of 28 subunits arranged into four stacked rings. The outer rings of the 20S Proteasome are composed of seven related but non-identical, non-catalytic subunits, alpha1-7, that form a gate and restrict substrate access. The inner rings of the 20S Proteasome are composed of seven related but non-identical subunits, beta1-7. beta1, 2, and 5 have proteyolytic activity. The 19S Proteasome caps one or both ends of the core particle and regulates substrate access to the catalytic core in an ATP-dependent manner by modulating 20S Proteasome conformation. The 19S Proteasome consists of a base subcomplex and a lid subcomplex. The base subcomplex is composed of six AAA+ family members, scaffolding proteins, and regulatory proteins involved in Ubiquitin recognition. The 19S Proteasome lid subcomplex contains eight subunits plus one deubiquitinating enzyme, Rpn11.

Additional 26S Proteasome Products

Product Documents for Human 26S Proteasome ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human 26S Proteasome ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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