24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62130

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Key Product Details

Sample Type & Volume Required Per Well

Cerebral spinal fluid, culture supernatants, tissue samples (100 uL)

Sensitivity

0.78 ng/ml

Assay Range

0.39-100ng/ml

View all 24(S)-Hydroxycholesterol ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 5.8% High Concentration %CV - 9.3%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 18.5% High Concentration %CV - 18.4%

Recovery for 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

Recovery

Cerebral Spinal Fluid - 99.0%
Brain Tissue Homogenate - 98.6%
TCM (serum free) - 95.5%
TCM (1% FBS) - 107.9%
TCM + 10% FBS - 101.6%

Scientific Data Images for 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: Non-species specific 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130] - Normal (non-diseased) human cerebral spinal fluid samples were diluted 1:2 in assay buffer and analyzed in the assay for 24(S)-Hydroxycholesterol levels. The blue bars represent the levels of 24(S)-Hydroxycholesterol in each sample as determined by ELISA.
ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: Non-species specific 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130] - Dose-response curves from human cerebral spinal fluid and rat brain tissue homogenate were diluted into assay buffer and compared to the 24(S)-Hydroxycholesterol standard curve. The parallel response indicates the standard effectively mimics the native mo
ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130]

ELISA: Non-species specific 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric) [NBP2-62130] - A typical standard curve for 24(S)-Hydroxycholesterol ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

  • Antibody
  • Assay Buffer
  • Conjugate
  • Microtiter Plate
  • Standard
  • Stop Solution 2
  • Streptavidin-HRP
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Product Documents for 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 24(S)-Hydroxycholesterol ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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