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Human 20S Proteasome ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-62173

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NBP2-62173

Key Product Details

Sample Type & Volume Required Per Well

Biological samples (100 uL)

Sensitivity

25 ng/mL

Assay Range

25 - 1600 ng/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Kit is not suitable for mouse or rat samples.

Recovery for Human 20S Proteasome ELISA Kit (Colorimetric)

Recovery

N/A

Scientific Data Images for Human 20S Proteasome ELISA Kit (Colorimetric)

ELISA: Human 20S Proteasome ELISA Kit (Colorimetric) [NBP2-62173] - The ELISA plate is coated with the capture antibody and the proteasome sample is added and allowed to complex with the bound antibody. The detection antibody is then added, followed by the secondary antibody. Subsequent reaction between the activated TMB

Kit Contents for Human 20S Proteasome ELISA Kit (Colorimetric)

  • Binding Buffer
  • Blocking Buffer
  • Capture Antibody
  • Conjugated Antibody
  • Detection Antibody
  • ELISA Buffer
  • Lysis Buffer
  • Plate
  • Proteasome Stock Solution
  • Stop Solution
  • TMB Substrate Solution
  • Wash Buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 20S Proteasome

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 Activator alpha.

Additional 20S Proteasome Products

Product Documents for Human 20S Proteasome ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human 20S Proteasome ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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