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Human 17-Beta-Estradiol ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # KA0297

Catalog #
Size / Price

Key Product Details

Sample Type & Volume Required Per Well

Saliva and Tissue culture media (100 uL)


28.5 pg/mL

Assay Range

29.3 to 30000 pg/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)




Quality control test: Standard curve. 17 beta-Estradiol ELISA Kit is a competitive immunoassay for the quantitative determination of 17 beta-Estradiol in biological and environmental samples.


Intra-Assay Precision (Precision within an assay) CV% < 8.60%

Inter-Assay Precision (Precision between assays) CV% < 6.20%

Recovery for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)


Cell Culture Supernatant: 92.20%, Saliva: 104.80%

Scientific Data Images for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)

ELISA: 17-Beta-Estradiol ELISA Kit [Alkaline Phosphatase] [KA0297] - Standard curve

Kit Contents for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)

  • 17 beta-Estradiol ELISA Antibody: A yellow solution of a rabbit polyclonal antibody to 17 beta-Estradiol.
  • 17 beta-Estradiol ELISA Conjugate: A blue solution of alkaline phosphatase conjugated with 17 beta-Estradiol.
  • 17 beta-Estradiol Standard: A solution of 300,000 pg/mL 17 beta-Estradiol.
  • Assay Buffer 3: Tris buffered saline containing proteins and detergents and sodium azide as a preservative
  • Goat anti-Rabbit IgG Microtiter Plate: A plate using break-apart strips coated with goat antibody specific to rabbit IgG.
  • Plate Sealer
  • pNpp Substrate: A solution of p-nitrophenyl phosphate in buffer. Ready to use.
  • Steroid Displacement Reagent: A special formulated displacer to inhibit steroid binding to proteins.
  • Stop Solution: A solution of trisodium phosphate in water. Keep tightly capped. Caution: Caustic
  • Wash Buffer Concentrate: Tris buffered saline containing detergents.

Preparation and Storage


The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: 17-Beta-Estradiol

17 beta Estradiol is a potent mammalian estrogenic hormone that is produced in the ovary, placenta, testis, and possibly the adrenal cortex.

Alternate Names

17 beta Oestradiol, 17-Beta-Estradiol, 17-beta-HSD

Additional 17-Beta-Estradiol Products

Product Documents for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.