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Lightning-Link (R) HRP Antibody Labeling Kit

Catalog # 701-0003 | Novus Biologicals a Bio-Techne Brand
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701-0003

Key Product Details

Applications

Immunohistochemistry, Western Blot

Kit Type

Antibody Labeling Kit

Conjugate

HRP

Product Summary for Lightning-Link (R) HRP Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our HRP antibody labeling kit enables the direct conjugation of Horseradish Peroxidase to any biomolecule with an available amine group. The researcher simply pipettes their antibody or other biomolecule into the vial of Lightning-Link HRP and incubates for 3 hours.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 5 vials, each suitable for labeling up to 4 mg of antibody.

Scientific Data Images for Lightning-Link (R) HRP Antibody Labeling Kit

Western Blot: Lightning-Link HRP Antibody Labeling Kit [701-0003]
Immunohistochemistry-Paraffin: Lightning-Link HRP Antibody Labeling Kit [701-0003]
Western Blot: Lightning-Link HRP Antibody Labeling Kit [701-0003]

Kit Contents for Lightning-Link (R) HRP Antibody Labeling Kit

  • 1 or 3 or 5 glass vial(s) of Lightning-Link mix
  • 1 vial of LL-Modifier reagent
  • 1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) HRP

Easy HRP labeling of your antibody with 30 seconds hands-on time. Antibodies are labeled to enzymes in order to amplify the signal via the catalytic properties of the enzyme. Horseradish peroxidase (HRP) labeling is one of the most commonly used enzymes for this purpose. HRP conjugates are ideal for use in ELISA, immunoblotting and immunohistochemistry applications. HRP has a faster catalytic rate than alkaline phosphatase (AP) and is more sensitive. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Product Documents for Lightning-Link (R) HRP Antibody Labeling Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) HRP Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Lightning-Link (R) HRP Antibody Labeling Kit (81)

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Customer Reviews for Lightning-Link (R) HRP Antibody Labeling Kit (4)

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  • Lightning-Link (R) HRP Antibody Labeling Kit
    Name: Anonymous
    Verified Customer | Posted 07/20/2020
    Used for labeling test article fortherapeutic drug.
    Lightning-Link (R) HRP Antibody Labeling Kit 701-0003
  • Name: Fugui Li
    Application: ELISA
    Sample Tested: purified monoclonall antibody
    Species: Human
    Verified Customer | Posted 09/23/2014
  • Name: Robert Hnasko
    Application: Western Blot
    Sample Tested: recombinant protein, Sample Amount: 100ng
    Species: Other
    Verified Customer | Posted 04/18/2011
    Lightning-Link (R) HRP Antibody Labeling Kit 701-0003
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified scFv protein
    Species: Human
    Verified Customer | Posted 06/10/2010

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FAQs for Lightning-Link (R) HRP Antibody Labeling Kit

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  • Q: What functional groups do I need on my protein?

    A: Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

  • Q: Do I need to purify the conjugate?

    A: No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

  • Q: Can non-antibody molecules be labeled?

    A: Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

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