DFHBI 1T

Product Description

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (K_d = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Solubility

Preparing Stock Solutions for DFHBI 1T

The following data is based on the product molecular weight 320.21

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.23 mL
5 mM	0.62 mL	3.12 mL	6.25 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Dey Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 2022 PMID: 34937909
• Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
• Filonov In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 2015 PMID: 26000751
• Svensen and Jaffrey Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 2016 PMID: 26877022
• Song Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 2014 PMID: 24393009