When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Mouse CD3+ T Cell Isolation Kit
Brand: R&D Systems
SKU: MAGM201
Price: 506 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Mouse CD3+ T Cell Isolation Kit

Catalog # MAGM201 | R&D Systems, Inc. a Bio-Techne Brand

Citations (10)

Product Datasheet / COA / SDS

Key Product Details

Setting the standard in quality research reagents

Technical Data
Citations (10) / Reviews / FAQs

Summary for MagCellect Mouse CD3+ T Cell Isolation Kit

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Product Specifications for MagCellect Mouse CD3+ T Cell Isolation Kit

Species

Mouse

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD3

Item	Value
Entrez Gene IDs	916 (Human); 12501 (Mouse)
Alternate Names	CD3, CD3 antigen, delta subunit, CD3-DELTA, CD3-GAMMA, CD3-epsilon, CD3G, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD_antigen: CD3e, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD3, CD3 antigen, delta subunit, CD3-DELTA, CD3-GAMMA, CD3-epsilon, CD3G, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD_antigen: CD3e, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE

Citations for MagCellect Mouse CD3+ T Cell Isolation Kit (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for MagCellect Mouse CD3+ T Cell Isolation Kit include:

Species: Mouse
Sample Types: Whole Cells

JH Choi et al. (2023-02-24), Single-cell transcriptome profiling of the stepwise progression of head and neck cancer Nature Communications, 2023-014(1):1055.
PMID: 36828832
Species: Mouse
Sample Types: Whole Cells

S Oh et al. (2023-01-19), Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells Nature Biotechnology, 2023-00(0).
PMID: 36658341
Species: Mouse
Sample Types: Tissue Homogenates

D Sridaran et al. (2022-11-14), Inhibiting ACK1-mediated phosphorylation of C-terminal Src kinase counteracts prostate cancer immune checkpoint blockade resistance Nature Communications, 2022-113(1):6929.
PMID: 36376335
Species: Mouse
Sample Types: Whole Cells

(2016-12-20), Intrinsic Photosensitivity Enhances Motility of T Lymphocytes Sci Rep, 2016-16(0):39479.
PMID: 27995987
Species: Mouse
Sample Types: Whole Cells

Khalid Z Matalka (2016-06-02), Eriobotrya japonica Water Extract Characterization: An Inducer of Interferon-Gamma Production Mainly by the JAK-STAT Pathway Molecules, 2016-021(6).
PMID: 27271577
Species: Mouse
Sample Types: Whole Cells

Badr M et al. (2014-02-07), Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12-interferon-gamma pathway. Onco Targets Ther, 2014-07(0):193-201.
PMID: 24532971
Species: Mouse
Sample Types: Whole Cells

Mutnal et al. (2013-07-31), T-cell reconstitution during murine acquired immunodeficiency syndrome (MAIDS) produces neuroinflammation and mortality in animals harboring opportunistic viral brain infection. J Neuroinflammation, 2013-010(1):98.
PMID: 23902750
Species: Mouse
Sample Types: Whole Cells

Wen L et al. (2010-12-08), hsBAFF regulates proliferation and response in cultured CD4(+) T lymphocytes by upregulation of intracellular free Ca(2+) homeostasis. Cytokine, 2010-153(2):215-22.
PMID: 21145253
Species: Mouse
Sample Types: Whole Cells

Purushothaman D et al. (2009-06-22), Cytokine-dependent regulation of NADPH oxidase activity and the consequences for activated T cell homeostasis. J. Exp. Med., 2009-0206(7):1515-23.
PMID: 19546249
Species: Mouse
Sample Types: Whole Cells

Bheeshmachar G et al. (2006-10-15), Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells. J. Immunol., 2006-1177(8):5041-50.
PMID: 17015687

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FAQs for MagCellect Mouse CD3+ T Cell Isolation Kit

When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Mouse CD3+ T Cell Isolation Kit

Catalog # MAGM201 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for MagCellect Mouse CD3+ T Cell Isolation Kit

Product Specifications for MagCellect Mouse CD3+ T Cell Isolation Kit

Preparation & Storage

Background: CD3

Citations for MagCellect Mouse CD3+ T Cell Isolation Kit (10)

FAQs for MagCellect Mouse CD3+ T Cell Isolation Kit

Product Documents for MagCellect Mouse CD3+ T Cell Isolation Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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