When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Human CD3+ T Cell Isolation Kit
Brand: R&D Systems
SKU: MAGH101
Price: 601 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Human CD3+ T Cell Isolation Kit

Catalog # MAGH101 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

Key Product Details

Setting the standard in quality research reagents

Technical Data
Citations (4) / Reviews / FAQs

Summary for MagCellect Human CD3+ T Cell Isolation Kit

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Product Specifications for MagCellect Human CD3+ T Cell Isolation Kit

Species

Human

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD3

Item	Value
Entrez Gene IDs	916 (Human); 12501 (Mouse)
Alternate Names	CD3, CD3 antigen, delta subunit, CD3-DELTA, CD3-GAMMA, CD3-epsilon, CD3G, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD_antigen: CD3e, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD3, CD3 antigen, delta subunit, CD3-DELTA, CD3-GAMMA, CD3-epsilon, CD3G, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD_antigen: CD3e, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE

Citations for MagCellect Human CD3+ T Cell Isolation Kit (4)

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Citations are publications that use Bio-Techne products. Selected citations for MagCellect Human CD3+ T Cell Isolation Kit include:

Species: Human
Sample Types: Whole Cells

Danesh A et al. (2013-12-12), Exosomes from red blood cell units bind to monocytes and induce proinflammatory cytokines, boosting T-cell responses in vitro. Blood, 2013-1123(5):687-96.
PMID: 24335232
Species: Human
Sample Types: Whole Cells

Zheng H et al. (2008-09-01), Mitogenic effect contributes to increased virulence of Streptococcus suis sequence type 7 to cause streptococcal toxic shock-like syndrome. Clin. Exp. Immunol., 2008-0153(3):385-91.
PMID: 18803762
Species: Human
Sample Types: Whole Cells

de la Fuente MA et al. (2007-01-09), WIP is a chaperone for Wiskott-Aldrich syndrome protein (WASP). Proc. Natl. Acad. Sci. U.S.A., 2007-0104(3):926-31.
PMID: 17213309
Species: Human
Sample Types: Whole Cells

Pillai V et al. (2006-12-19), Transient regulatory T-cells: a state attained by all activated human T-cells. Clin. Immunol., 2006-1123(1):18-29.
PMID: 17185041

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Showing 1 - 4 of 4 citations Showing All

FAQs for MagCellect Human CD3+ T Cell Isolation Kit

When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Human CD3+ T Cell Isolation Kit

Catalog # MAGH101 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for MagCellect Human CD3+ T Cell Isolation Kit

Product Specifications for MagCellect Human CD3+ T Cell Isolation Kit

Preparation & Storage

Background: CD3

Citations for MagCellect Human CD3+ T Cell Isolation Kit (4)

FAQs for MagCellect Human CD3+ T Cell Isolation Kit

Product Documents for MagCellect Human CD3+ T Cell Isolation Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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